General Cell Based Assays
AURORA BIOLABS LLC Kras Q61K Nucleotide Exchange Assay Kit,100 rxn
KRAS is a small GTPase in the RAS protein family that plays a critical role in regulating cell signaling pathways involved in proliferation, differentiation, and survival. KRAS cycles between an inactive GDP-bound state and an active GTP-bound state, and activation occurs through nucleotide exchange from GDP to GTP. Mutations such as KRAS Q61K are frequently associated with human cancers and contribute to abnormal signaling and uncontrolled cell growth. The KRAS Q61K Nucleotide Exchange Assay is a TR-FRET–based assay designed to quantitatively monitor nucleotide exchange activity of KRAS Q61K. The assay detects changes in fluorescence resonance energy transfer generated during nucleotide exchange, enabling measurement of KRAS activation and evaluation of compounds that modulate KRAS signaling for cancer research and drug discovery applications.
Cayman Chemical Cysteinyl Leukotriene Express
Cayman’s CysLT Express ELISA Kit is a competitive assay that can be used for quantification of CysLTs in urine, plasma, serum, lavage fluids and aspirates, culture media, and other sample matrices. The assay has a range of 7.8-1,000 pg/ml, an average sensitivity (80% B/B0) of 24 pg/ml, and a lower limit of detection (LLOD) of 6.4 pg/ml. NOTE: This kit measures all CysLTs. For measurement of specific CysLTs, please see Cayman Chemical’s Leukotriene E4 ELISA Kit (Item No. 501060) and Leukotriene C4 ELISA Kit (Item No. 501070).
Eurofins Discoverx PathHunter eXpress CCR7 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0195E2CP0L)
The PathHunter CCR7 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL19/MIP-3β) based activation of CCR7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR7 and EA-tagged β-Arrestin-2. Activation of CCR7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress EpoR/EpoR Dimerization Assay, 2-Plate Kit. (93-0965E3CP0M)
The PathHunter EpoR/EpoR Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. erythropoietin) induced dimerization of EpoR/EpoR receptors. This product uses EFC technology. The cell line included overexpresses EpoR target protein fused to PK alongside EpoR target protein fused to EA. Upon ligand-induced dimerization of EpoR/EpoR, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Human G-CSF ASY 25 PL
The Meso Scale Discovery U-PLEX Human G-CSF Assay is a multiplex immunoassay kit that enables simultaneous quantitative measurement of Granulocyte Colony Stimulating Factor (G-CSF) in human serum plasma and cell culture supernatants It utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision with minimal matrix interference This assay requires 50 L or less sample volume and can be read on MSD instrument platforms It includes a plate coated with capture antibodies detection antibodies and calibrators and controls needed to generate a standard curve and measure G-CSF concentrations precisely
ABclonal Technology LDH-B
This gene encodes the B subunit of lactate dehydrogenase enzyme which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD in a post-glycolysis process Alternatively spliced transcript variants have been found for this gene Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism and that this isoform is localized in the peroxisomes Mutations in this gene are associated with lactate dehydrogenase B deficiency Pseudogenes have been identified on chromosomes X 5 and 13
Eurofins Discoverx PathHunter Human GPR85 β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Human GPR85 β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure GPR85 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Meso Scale Discovery R-PLEX Human Cathepsin L2 ASY
The R-Plex Human Cathepsin L2 Assay is a multiplex immunoassay kit that enables simultaneous quantitative measurement of human Cathepsin L2 protein in cell and tissue lysates It uses a quantitative sandwich immunoassay technique and color-coded magnetic beads for multiplex analysis The assay has a detection range of 78-50 000 pg/mL and includes a 96-well plate assay buffer wash buffer serum matrix standards detection antibodies streptavidin-PE and magnetic beads It can help researchers study Cathepsin L2 s role in cancer bone remodeling and protein processing The assay requires a Luminex-based reader and xPONENT software for analysis
ABclonal Technology ADAM17 Rabbit pAb
This gene encodes a member of the ADAM (a disintegrin and metalloprotease) family, which includes membrane-anchored proteins related to snake venom disintegrins. These proteins are involved in various biological processes such as fertilization, muscle development, and neurogenesis. The preproprotein is processed to produce the mature protease, which sheds the ectodomain of tumor necrosis factor-alpha (TNF-alpha), releasing soluble TNF-alpha. This protease also processes cell adhesion proteins, cytokine and growth factor receptors, and EGF receptor ligands, and activates the Notch signaling pathway. Elevated expression of this gene has been linked to psoriasis, rheumatoid arthritis, multiple sclerosis, and Crohn’s disease, suggesting a role in autoimmune diseases. Additionally, the protease may play a role in viral infection by cleaving ACE2, the receptor for SARS-CoV and SARS-CoV-2.
Eurofins Discoverx PathHunter eXpress GLP2R CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0572E2CP2M)
The PathHunter GLP2R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. GLP-2) based activation of GLP2R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP2R and EA-tagged β-Arrestin-2. Activation of GLP2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Crystal Chem Inc Rat Glucose Assay Kit, 10-Kit Pack, 96-tests per well
The Rat Glucose 10-kit pack is an economical option for high-volume users of our Rat Glucose Assay Kit offered as ten individual kits.Highlights: Easy-to-use, results in 5 minutes. Kits use a sample size of only 2 µL. For use with serum, plasma, and urine. Designed for rat samples. Crystal Chem is a worldwide leading supplier of mouse and rat ELISA kits for research use. Our kits are among the highest quality found in the market, and are used by leading research universities, pharma companies, and government institutions around the world.
Eurofins Discoverx PathHunter BDKRB2 Bioassay Kit, 10-Plate. (93-0189Y2-00114)
The PathHunter BDKRB2 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. bradykinin) based activation of BDKRB2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged BDKRB2 and EA-tagged β-Arrestin-2. Activation of BDKRB2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
ABclonal Technology OPRK1 Rabbit pAb
This gene encodes an opioid receptor, which is a member of the 7 transmembrane-spanning G protein-coupled receptor family. It functions as a receptor for endogenous ligands, as well as a receptor for various synthetic opioids. Ligand binding results in inhibition of adenylate cyclase activity and neurotransmitter release. This opioid receptor plays a role in the perception of pain and mediating the hypolocomotor, analgesic and aversive actions of synthetic opioids. Variations in this gene have also been associated with alcohol dependence and opiate addiction. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon.
Eurofins Discoverx PathHunter Human GPR101 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR101 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR101 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human PROKR1 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human PROKR1 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure PROKR1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.